产品编号 30-13721r
英文名称 cdhf14
中文名称 钙粘蛋白14抗体
别 名 cadherin family member 14; cdhf14; fat tumor suppressor homolog 4; fat-like cadherin protein fat-j; fat4; fat4_human; fatj; hfat4; nbla00548; protocadherin fat 4.
说 明 书 0.1ml 0.2ml
研究领域 肿瘤 细胞生物 信号转导 细胞粘附分子 细胞类型标志物
抗体来源 rabbit
克隆类型 polyclonal
交叉反应 human, mouse, rat, dog, pig, cow, horse, sheep,
产品应用 elisa=1:500-1000 ihc-p=1:100-500 ihc-f=1:100-500 icc=1:100-500 if=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 538kda
细胞定位 细胞膜
性 状 lyophilized or liquid
浓 度 1mg/1ml
免 疫 原 klh conjugated synthetic peptide derived from human cdhf14
亚 型 igg
纯化方法 affinity purified by protein a
储 存 液 0.01m tbs(ph7.4) with 1% bsa, 0.03% proclin300 and 50% glycerol.
保存条件 store at -20 °c for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°c. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody the antibody is stable for at least two weeks at 2-4 °c.
pubmed pubmed
产品介绍 background:
the cadherins represent a family of ca2+-dependent adhesion molecules that function to mediate cell to cell binding that is critical for the maintenance of structure and morphogenesis. cadherins contain a large extracellular domain at the n-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. the relatively short c-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. the cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. fat4 (fat tumor suppressor homolog 4), also known as fat-j, cdhf14 or cdhr11, is a 4,981 amino acid single-pass type i membrane protein that belongs to the protocadherin subfamily of cadherins and localizes to the primary cilia of kidney. widely expressed, fat4 contains thirty-four cadherin domains, six egf-like domains and two laminin g-like domains. fat4 may function in the regulation of planar cell polarity.
function:
may function in the regulation of planar cell polarity (by similarity). cadherins are cell-cell interaction molecules.
subcellular location:
membrane. in the kidney, localizes to primary cilia.
tissue specificity:
widely expressed. expressed in fetal brain, infant brain, brain tumor and colorectal cancer.
similarity:
contains 34 cadherin domains.
contains 6 egf-like domains.
contains 2 laminin g-like domains.
gene id:
79633
database links:
entrez gene: 79633 human
swissprot: q6v0i7 human
unigene: 563205 human
important note:
this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片 cell: (mo)brain(2% paraformaldehyde fixed for 10 minutes, 90% methanol permeablized for 10 minutes).
concentration:1:25.
incubation: 40 minutes.
host/blank: (mo)brian cells.
flow cytometric analysis of rabbit anti-cdhf14 antibody (bs-13721r)(green) compared with control in the absence of primary antibody (blue) followed by mouse brain cells.
secondary antibody: goat anti-rabbit igg/fitc antibody (bs-0295g-fitc).
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